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Consistency matters more than intensity, since steady glutathione levels are what sustain the brightening effect over time

Centre of Research in Epidemiology and Statistics

Determination of Nitric Oxide (NO) Production The nitrite (NO 2 ) was estimated colorimetrically at 12 and 24 h post CLP on the basis of reduction of nitrate to nitrite using Griess method ( Bacterial Load Determination For determination of colony-forming units (CFU) in blood and peritoneal fluid of mice ( n = 6 per/group), 10 L of samples were diluted with sterile Phosphate-Buffered Saline (PBS) 1:10, and then 10 L of each dilution was cultured in Mller Hinton Agar and incubated at 37 C for 24 h

HL60 cells were differentiated into a neutrophil-like cell line (dHL60) by maintaining cells in media containing 1% DMSO for 5 days